BMG high-throughput screening instrument-Fornitore globale

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BMG high-throughput screening instrument

The full wavelength and full function enzyme-linked immunosorbent assay (ELISA) reader, one of the new members of the BMGLABTECH family, was launched

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Product details

Purpose:

High throughput rapid testing in cellular and molecular biology, chemistry, immunology, physiology, pharmacology, and other fields,

Intermolecular interactions,

Report genes,

Enzyme activity and kinetic studies,

ATPContent determination,

Cell proliferation, cytotoxicity,PCRQuantitative,

Membrane potential, ion channels, signal transduction, etc.

Main features:

Can be used for full wavelength fluorescence intensity, time-resolved fluorescence, UV visible absorption light（Continuous wavelength scanning）Detection of chemiluminescence and bioluminescence;
Fluorescence polarization test
Alyha test
Top and bottom two reading modes
Two sets of samplers can be equipped as needed, with a pipetting range of5~350 μl，0.5μLIncremental, each hole can add different volumes, and each hole can add up to4Secondary sample
45℃incubation（selectable60℃, and can be placed in a cold room for low-temperature detection）
Slow and Fast Dynamics—The fastest speed can reach every second50Secondary test reading
Can be doneBRET / FRETtest
It can simultaneously perform fluorescence and luminescence dual emission optical reading, and dual excitation detection is currently the fastest among similar brandsFRETperhapsBRETFluorescence intensity measurement analyzer

CLARIOstar is a modular, full wavelength, and fully functional enzyme-linked immunosorbent assay (ELISA) reader that combines advanced LVF monochromator, high-sensitivity filter technology, and ultrafast spectrophotometer technology. It has 8 different detection modes, including: FI fluorescence intensity, FRET, Fluorescence polarization luminescence, BRET, UV/Vis absorption, time-resolved fluorescence, TR-FRET, And AlphaScreen ® / AphaLISA ®.

characteristic
Full spectrum UV/visible absorbance (220 to 1000nm) and BMG's unique ultrafast spectrometer (<1 second/well); AlphaScreen and AlphaLISA detection have high-energy lasers, kinetics, and cell analysis that can accommodate reagent injection. The integrated fluorescence library makes it easier to conduct experimental development.

Advanced LVF monochromator and filter selection system
*The measurement and development of fluorescence detection methods, CLARIOstar has an advanced monochromator flexibility that can be combined with higher sensitivity filters. Advanced monochromators with continuously adjustable wavelengths (320 to 850nm) and bandwidths (8 to 100nm) for excitation and emission,Compared to traditional monochromatorsSignificantly enhanced performance.

The technical parameters are as follows:

test method	visible light/UV Absorption Spectra
	Fluorescence intensity（FI）Including FRET
	Time-resolved fluorescence（TRF）Including TR-FRET
	AlphaScreen ®
	polarization
	Glowing（FlashandGlow）Including BRET
detection mode	Top and bottom detection
	Endpoint and dynamic testing
	Continuous dual excitation luminescence detection
	Continuous dual emission detection
	Spectral scanning
	Pore scanning
Read Mode	Top and bottom reading
light source	High intensity flashing xenon lampAlphaScreen for high-speed lasers ® / AlphaLISA ®
detector	Low noise photomultiplier tube and CCD spectrometer
Wavelength selection	Linear Variable Filter (LVF) Monochromator:Separate excitation and emission using linearly variable bidirectional colors
	Filter: 4 excitation and 4 emission filters each
	LVF monochromator+filter: Ex 1, Em Other
wavelength range	240 - 750 nm or 240 - 900 nm for FI, FP, FRET, TRF, TR-FRET （filters） 320 - 850 nm for FI, FRET, TRF （monochromators） 240 - 750 nm for LUM （filters） 320 - 750 nm for LUM （monochromator） 220 - 1000 nm for ABS
light path	Top and bottom: Top and bottom: enclosed, with motor-driven mirrors and dichroic guidance to freely switch optical paths
Z-axis adjustment	Automatic focus height adjustment (0.1 mm resolution)
injector	The biggest2A built-in sampler
	compatible384Orifice plate, injection at measurement position
	The injection volume of each detection hole can be adjusted（3…350μL）
	Adjustable injection speed（100…420μL/s）
	Each hole can be set as many as possible4Different injection volumes
	Reagent recycling function
incubator	Room temperature+5℃ to45℃（65℃ optional)
	Temperature Stability<0.2℃
	Temperature uniformity<0.5℃
oscillating	Linear, track, and double track, custom time and speed
Microplate type	6…1536Orifice plate, custom plate，
Absorption spectrophotometer	wavelength range	220nm…1000nm
	Wavelength Resolution	1nm
	ODValue Range	0…4
	accuracy	2ODplace<0.1%
	accuracy	1ODplace<0.5% 2ODplace<0.8%
sensitivity	FI filter (top): 0.4 pM （< 8 amol/well Fluorescein, 384sv, 20 μL） FI filter (bottom): 1.0 pM (<50 amol/well Fluorescein, 384g, 50 μ L) FI monochromator (top): 0.5 pM (<10 amol/well Fluorescein, 384sv, 20 μ L) FI monochromator (top): 4.0 pM (<200 amol/well Fluorescein, 384g, 50 μ L) FP： 1.0 mP SD at 1 nM Fluorescein （384sv, 20 μL） TRF：0.1 pM （< 2 amol/well Europium, 384sv, 20 μL） LUM：0.75 pM （< 15 amol/well ATP, 384sv, 20 μL） AlphaScreen ® ： 6 pM （< 100 amol/well P-Tyr-100, 384sv, 17 μL） ABS spectrophotometer: Wavelength range: 220 - 1000 nm Full wavelength scanning<1 s/well Optional wave width: 1, 2, 5, and 10 nm OD range: 0 - 4 OD Accuracy:< 1% at 2 OD Accuracy:< 0.5% at 1 OD and < 0.8% at 2 OD
Reading board time	Flyingmode	9s（96Orifice plate) 14s（384Orifice plate) 27s1536
software	Integrated fluorescence image library, multi-user control software, and MARS statistical analysis software, includingFDA 21 CFR Part 11 compliant
volume	Width:45cm/Long:51cm/High:40cm
weight	32kg
Stack IIAutomatic board feeder	Zui can automatically inject samples50Block microporous plate
	Continuous board entry mode
	Bar code reader
Microplate incubator	Incubation and oscillation of microplates with 96 or more holes
optical filter	The optimized fluorescence staining can be used for special analysis Filter can detect anything including UV to NIR Customer customized filter
upgrade	It can be easily upgraded, such as detection mode and reagent injection
	LOD = 3 x SD （20 blanks） / slope （6 pt std curve） AlphaScreen ® P-Tyr-100 assay kit, PerkinElmer, #6760620C Microplatets: white for LUM, AlphaScreen ®, TRF; black for FI, FP; clear for ABS 96 = 96-well microplates 384sv = 384-well small volume microplates 384g = 384-well glass bottom microplates 1536 = 1536-well microplates

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